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  • Buffer solution configuration for 0.5 M EDTA and 1 M DTT

    Buffer solution configuration for 0.5 M EDTA and 1 M DTT

    Buffer solution configuration for 0.5 M EDTA and 1 M DTT Measuring Beakers,Glass Beaker 1000ml,Wholesale Glass Beakers (1) 0.5M EDTA (pH8.0) Component concentration: 0.5 M EDTA Preparation volume: 1 L Preparation method: Weigh out 186.1 g of Na2EDTA·2H2O and place it in a 1 L beaker. 2. Add about...
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  • Plasmid extraction method

    Plasmid extraction method

    Plasmid extraction method Solution I (for plasmid extraction) Component concentrations: 25 mM Tris-HCl (pH 8.0), 10 mM EDTA, 50 mM Glucose Preparation volume: 1 L Preparation method: 1. Measure the following solutions into a 1 L beaker. 1M Tris-HCl (PH8.0) 25ml 0.5M EDTA (PH8.0) 20ml 20% G...
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  • Operation steps for graduated centrifuge tubes

    Operation steps for graduated centrifuge tubes

    Operation steps for graduated centrifuge tubes 1. Because the graduated centrifuge tube is a measuring device, it must be cleaned and dried before use. 2. When reading, be sure to observe the meniscus correctly, otherwise certain errors will be introduced. 3. When using, the number of centrifuge ...
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  • Classification of graduated centrifuge tubes

    Classification of graduated centrifuge tubes

    Classification of graduated centrifuge tubes There are two types of graduated centrifuge tubes: conical bottom and round bottom. The conical centrifuge tube can be divided into two types with plug and no plug. The Conical centrifuge tubes is a conical-bottom glass tube with a round mouth on the u...
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  • HCl and Glucose Buffer Configuration Scheme

    HCl and Glucose Buffer Configuration Scheme

    HCl and Glucose Buffer Configuration Scheme 1) 2.5N HCl Component concentration: 2.5 N HCl Preparation amount: 100 ml Preparation method: 1. Add 21.6 ml of concentrated hydrochloric acid (11.6 N) to 78.4 ml of deionized water and mix well. 2. Store at room temperature. 2) 5M NaCl Component concen...
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  • SDS and NaOH buffer configuration scheme

    SDS and NaOH buffer configuration scheme

    SDS and NaOH buffer configuration scheme (1) 10% (W/V) SDS Component concentration: 10% (W/V) SDS Preparation amount: 100ml Preparation method: 1. Weigh 10g of high-purity SDS into a 100-200ml beaker, add about 80ml of deionized water, and dissolve it at 68°C 2. Add concentrated hydrochloric acid...
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  • Determination of Antibiotic Potency by Tube and Disk Method

    Determination of Antibiotic Potency by Tube and Disk Method

    Determination of Antibiotic Potency by Tube and Disk Method [Purpose and Requirements] 1. Understand the principle of the tube-dish method. 2. Master the technique and calculation method of the two-dose method to determine the antibiotic titer unit. [Principle] The bioassay of antibiotics is ...
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  • Ammonium acetate/phenol/chloroform/isoamyl alcohol buffer configuration scheme

    Ammonium acetate/phenol/chloroform/isoamyl alcohol buffer configuration scheme

    Ammonium acetate/phenol/chloroform/isoamyl alcohol buffer configuration scheme 10 M Ammonium Acetate Component Concentration: 10 M Ammonium Acetate Preparation amount: 100 ml Preparation method: 1. Weigh 77.1 g of ammonium acetate into a 100-200 ml beaker, add about 30 ml of deionized water, ...
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  • 3 M sodium acetate and PBS Buffer buffer configuration scheme

    3 M sodium acetate and PBS Buffer buffer configuration scheme

    3 M sodium acetate and PBS Buffer buffer configuration scheme 1) 3 M sodium acetate (pH 5.2) Component concentration: 3M sodium acetate Preparation amount: 100ml Preparation method: 1. Weigh 40.8g NaAc·3H 2 O into a 100-200ml beaker, add 40ml of deionized water and stir to dissolve 2. Add glacial...
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