Industry News
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10×TE Buffer and 1.5 M Tris-HCl buffer configuration scheme
10×TE Buffer and 1.5 M Tris-HCl buffer configuration scheme 1) 10×TE Buffer (pH7.4, 7.6, 8.0) Component concentrations: 100 mM Tris-HCl, 10 mM EDTA Preparation volume: 1 L Preparation method: 1. Measure the following solutions into a 1 L beaker. 1M Tris-HCl Buffer (PH7.4, 7.6, 8.0) 100ml ...Read more -
Tris-HCl solution configuration method
Tris-HCl solution configuration method 1 M Tris-HCl (pH 7.4, 7.6, 8.0) Component concentration: 1 M Tris-HCl Preparation volume: 1 L Preparation method: 1. Weigh 121.1 g of Tris into a 1 L beaker. 2. Add about 800 ml of deionized water and stir well to dissolve. 3. Add concentrated hydrochloric a...Read more -
Extraction of pectin and preparation of jelly
Extraction of pectin and preparation of jelly 【Purpose】 Master the principle of pectin extraction and learn the technology of jelly preparation. 【Principle of experiment】 Pectin is a macromolecular carbohydrate compound, a natural colloidal substance of plant origin, which is widely present i...Read more -
Immunohistochemical experiments on mouse tissue sections
Immunohistochemical experiments on mouse tissue sections 1. Bake the paraffin sections in a 60°C oven overnight. 2. Dewax in xylene, add gradient alcohol into water (anhydrous ethanol, 95% alcohol), soak in distilled water for later use. 3. Antigen retrieval Take 500ml of EDTA antigen retrieval w...Read more -
How to prove the existence of pinhole edge effect
How to prove the existence of pinhole edge effect Glass Beakers Clear,Graduated Beaker,Science Beaker Principle Stomata transpiration is the main way for plants to lose water. The stomata are very small, and their total area generally does not exceed 1% of the leaf area. %, such an amazing amount...Read more -
ZB-1C and ZBS-6E disintegration apparatus operating procedures
ZB-1C and ZBS-6E disintegration apparatus operating procedures 1. Preparation Put salt-free water in the water bath to the indicated mark. 1.2 Put the borosilicate beaker containing the desired solution into the water bath. 1.3 Hang the hanging basket on the metal bracket, adjust the water level,...Read more -
Two-dimensional electrophoresis: cleaning the IEF tube
1. After IEF, thoroughly clean the tube with deionized water. 2. Heat 500ml 0.6% Deconex solution to 60~80°C. 3. Immerse the glass tube in the Deconex solution and place it at 70°C for 3 times for 10 minutes: After every 10 minutes, remove the glass tube with tweezers, pour out the cleaning solut...Read more -
How to prove the existence of the edge of the small hole
[Principle] Stomatal transpiration is the main way for plants to lose water. The stomata are very small, and their total area generally does not exceed 1% of the leaf area. However, the water lost by the leaf through stomata transpiration reaches 50 80% of the free surface equal to the leaf area....Read more -
Fluorescein FITC-labeled antibody method
1. Marsshall method (1) Material antibody globulin solution, 0.5 mol/L (pH 9.0) carbonate buffer, sterile saline, fluorescein isothiocyanate, 1% thimerosal aqueous solution, 50ml beaker, 4℃ refrigerator, Electromagnetic stirrer, dialysis bag, glass rod, 0.01mol/LPBS with pH 7.2 or 3.0, etc. (2) M...Read more
