Ammonium acetate/phenol/chloroform/isoamyl alcohol buffer configuration scheme
10 M Ammonium Acetate
Component Concentration: 10 M Ammonium Acetate
Preparation amount: 100 ml
Preparation method:
1. Weigh 77.1 g of ammonium acetate into a 100-200 ml beaker, add about 30 ml of deionized water, and stir to dissolve.
2. Add deionized water to bring the solution to 100 ml.
3. Sterilize by filtration through a 0.22 mm filter.
4. Seal the bottle and store at room temperature.
Note: Ammonium acetate is easily decomposed by heat, so it cannot be autoclaved.
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Phenol/chloroform/isoamyl alcohol (25 : 24 : 1)
Preparation method:
1. Instructions: Phenol/chloroform/isoamyl alcohol (25 : 24 : 1) is often used for protein removal from nucleic acid samples. Chloroform denatures proteins and aids in the separation of the liquid phase from the organic phase, while isoamyl alcohol helps eliminate air bubbles during extraction.
2. Preparation method: Mix Tris-HCl equilibrated phenol with an equal volume of chloroform/isoamyl alcohol (24:1), and then transfer to a brown glass bottle and store at 4°C.
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Post time: Feb-14-2022
