Welcome To Huida

Plasmid extraction method

Solution I (for plasmid extraction)

Component concentrations: 25 mM Tris-HCl (pH 8.0), 10 mM EDTA, 50 mM Glucose

Preparation volume: 1 L

Preparation method: 1. Measure the following solutions into a 1 L beaker.

2. After autoclaving, store at 4°C.

3. Add 2 ml of RNase A (20 mg/ml) to every 50 ml of Solution I before use.

Solution II (for plasmid extraction)

Component concentration: 200mM NaOH, 1% (W/V) SDS

Preparation amount: 500ml Preparation method:

1. Measure the following solutions and place them in a 500ml beaker

10% SDS 50ml

2N NaOH 50ml

2. Add sterilized water to 500ml and mix well

3. Store at room temperature, this solution should not be stored for more than one month

Note: SDS is prone to bubbles, do not stir vigorously

Solution III (for plasmid extraction)

Component concentrations: 3 M KOAc, 5 M CH3COOH

Preparation volume: 500 ml Preparation method:

1. Weigh the following reagents into a 500 ml beaker.

KOAc 147g

CH3COOH 57.5ml

2. Add 300 ml of deionized water and stir to dissolve.

3. Add deionized water to bring the solution to 500 ml.

4. After autoclaving, store at 4°C.

Dedicated to strict quality manage and thoughtful buyer company, our experienced workers members are generally available to discuss your specifications and make certain full consumer gratification for 1000ml Transparent Glass Beaker, China beakers borosilicate glass glassware, Heat Resistant Glass Beakers, We have been proud to supply our goods to every labwares all around the world with our flexible, fast efficient services and strictest quality control standard which has always approved and praised by customers.