Tris-HCl solution configuration method
1 M Tris-HCl (pH 7.4, 7.6, 8.0)
Component concentration: 1 M Tris-HCl
Preparation volume: 1 L
Preparation method:
1. Weigh 121.1 g of Tris into a 1 L beaker.
2. Add about 800 ml of deionized water and stir well to dissolve.
3. Add concentrated hydrochloric acid according to the following table to adjust the desired pH value.
| pH | Concentrated HCl |
| 7.4 | about 70ml |
| 7.6 | about 60ml |
| 8.0 | about 42ml |
4. Make up the solution to 1 L.
5. After autoclaving, store at room temperature.
Note: The pH value of the solution should be cooled to room temperature before adjusting the pH value, because the pH value of the Tris solution varies greatly with temperature, and the pH value of the solution decreases by approximately 0.03 units for every 1°C increase in temperature.
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Post time: Feb-08-2022
