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10×TE Buffer and 1.5 M Tris-HCl buffer configuration scheme

1) 10×TE Buffer (pH7.4, 7.6, 8.0)
Component concentrations: 100 mM Tris-HCl, 10 mM EDTA
Preparation volume: 1 L
Preparation method:
1. Measure the following solutions into a 1 L beaker.

2. Add about 800 ml of deionized water to the beaker and mix well.
3. After the solution is made up to 1 L, autoclave.
4. Store at room temperature.

2) 1.5M Tris-HCl (pH8.8)
Component concentration: 1.5 M Tris-HCl
Preparation volume: 1 L
Preparation method:
1. Weigh 181.7 g of Tris into a 1 L beaker.
2. Add about 800 ml of deionized water and stir well to dissolve.
3. Adjust pH to 8.8 with concentrated hydrochloric acid.
4. Make up the solution to 1 L.
5. After autoclaving, store at room temperature.
NOTE: The pH should be adjusted after allowing the solution to cool to room temperature, as the pH of Tris solutions varies greatly with temperature, with each increase in temperature
At 1°C, the pH of the solution decreases by approximately 0.03 units.

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