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SDS and NaOH buffer configuration scheme
SDS and NaOH buffer configuration scheme (1) 10% (W/V) SDS Component concentration: 10% (W/V) SDS Preparation amount: 100ml Preparation method: 1. Weigh 10g of high-purity SDS into a 100-200ml beaker, add about 80ml of deionized water, and dissolve it at 68°C 2. Add concentrated hydrochloric acid...Read more -
Determination of Antibiotic Potency by Tube and Disk Method
Determination of Antibiotic Potency by Tube and Disk Method [Purpose and Requirements] 1. Understand the principle of the tube-dish method. 2. Master the technique and calculation method of the two-dose method to determine the antibiotic titer unit. [Principle] The bioassay of antibiotics is ...Read more -
Ammonium acetate/phenol/chloroform/isoamyl alcohol buffer configuration scheme
Ammonium acetate/phenol/chloroform/isoamyl alcohol buffer configuration scheme 10 M Ammonium Acetate Component Concentration: 10 M Ammonium Acetate Preparation amount: 100 ml Preparation method: 1. Weigh 77.1 g of ammonium acetate into a 100-200 ml beaker, add about 30 ml of deionized water, ...Read more -
3 M sodium acetate and PBS Buffer buffer configuration scheme
3 M sodium acetate and PBS Buffer buffer configuration scheme 1) 3 M sodium acetate (pH 5.2) Component concentration: 3M sodium acetate Preparation amount: 100ml Preparation method: 1. Weigh 40.8g NaAc·3H 2 O into a 100-200ml beaker, add 40ml of deionized water and stir to dissolve 2. Add glacial...Read more -
10×TE Buffer and 1.5 M Tris-HCl buffer configuration scheme
10×TE Buffer and 1.5 M Tris-HCl buffer configuration scheme 1) 10×TE Buffer (pH7.4, 7.6, 8.0) Component concentrations: 100 mM Tris-HCl, 10 mM EDTA Preparation volume: 1 L Preparation method: 1. Measure the following solutions into a 1 L beaker. 1M Tris-HCl Buffer (PH7.4, 7.6, 8.0) 100ml ...Read more -
Tris-HCl solution configuration method
Tris-HCl solution configuration method 1 M Tris-HCl (pH 7.4, 7.6, 8.0) Component concentration: 1 M Tris-HCl Preparation volume: 1 L Preparation method: 1. Weigh 121.1 g of Tris into a 1 L beaker. 2. Add about 800 ml of deionized water and stir well to dissolve. 3. Add concentrated hydrochloric a...Read more -
Extraction of pectin and preparation of jelly
Extraction of pectin and preparation of jelly 【Purpose】 Master the principle of pectin extraction and learn the technology of jelly preparation. 【Principle of experiment】 Pectin is a macromolecular carbohydrate compound, a natural colloidal substance of plant origin, which is widely present i...Read more -
Immunohistochemical experiments on mouse tissue sections
Immunohistochemical experiments on mouse tissue sections 1. Bake the paraffin sections in a 60°C oven overnight. 2. Dewax in xylene, add gradient alcohol into water (anhydrous ethanol, 95% alcohol), soak in distilled water for later use. 3. Antigen retrieval Take 500ml of EDTA antigen retrieval w...Read more -
How to prove the existence of pinhole edge effect
How to prove the existence of pinhole edge effect Glass Beakers Clear,Graduated Beaker,Science Beaker Principle Stomata transpiration is the main way for plants to lose water. The stomata are very small, and their total area generally does not exceed 1% of the leaf area. %, such an amazing amount...Read more
