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Preparations for cardiomyocyte culture

【Preparation matters】
Preparation: Hanks solution, low-sugar DMEM solution, high-sugar DMEM solution, trypsin digestion solution, double antibody solution, sodium bicarbonate solution, hydrochloric acid solution.
Take the rat: Spread the rat basin with cotton and take the rat.
Preparation: Check beforehand whether the microscope, magnetic stirrer and centrifuge are easy to use. Take out the calf serum, pancreatin, and antibiotics three hours in advance. The rest of the unfrozen medicines were also taken out 20 minutes in advance to relax. Synermide (0.1%) foam gauze (use 10ml 5% of Synermide to add water to 500ml), disinfect the ground (1:500 84 liquids).
Wipe the table with an alcohol cotton ball and place the objects. Turn on the ultraviolet light for 30 minutes and then turn on the blower to blow until the end of the experiment.
Items: White coat, lunch box
Small scissors, small tweezers (4 sets) (one set to cut the skin, one set to open the chest to remove the heart, one set to remove the fibrous tissue of the heart border, one set to cut the heart)
Bottle dishes (2 sets) [Two small round petri dishes, add DMEM solution in the petri dishes, and then fill the removed heart and the heart after removing the fibrous tissue] [A small beaker contains iodine and alcohol cotton balls]
3 250ml beakers [one for water bath, add water in advance, preferably one or two steaming] [one containing about 150ml of 0.1% neogermide, sterilize mice] [ glass lab beaker,beakers glassware,beaker 2000ml,laboratory beaker ]
One 500ml beaker is used as a waste liquid tank.
3 50ml beakers (cut the myocardial tissue, and finally dispense liquid)  [ glass lab beaker,beakers glassware,beaker 2000ml,laboratory beaker ]
Conical flask(50ml) + small rotor (small rotor, rubbed with alcohol, rinsed with double distilled water, then rinsed out the alcohol thoroughly, and then pressurized with a conical flask)  [ conical flask 250ml erlenmeyer,5ml -5000ml conical flask,100ml conical flask,conical flask ]
Centrifuge tube and cap (12-14), two test tubes
Straws (5~8) large tweezers (two handles, holding objects, cotton ball, etc.)
Countertop: magnetic stirrer, gauze (preliminarily used to soak the tray with 0.1% neogermide), test tube rack, foam board, five needles (not sterilized) 75% alcohol cotton ball and iodine (sterilized cotton ball, soaked in alcohol, iodine) Wine) Two large tweezers (one cotter ball, the other to take the mouse), 3 250ml flasks (one for distilled water, one for neocerin, and the other empty spare) and one 500ml flask (waste tank) , Thermometer, PH test paper (pre-adjust the pH value), alcohol lamp, lighter/match, microscope glass slide (5-10 pieces) [used to check the inoculation density before culturing and check the cell density after digestion], 6 syringes 5ml (pancreas) Enzyme, DMEM, calf serum) 1ml 2 (double-antibody liquid) 20ml spare 2 and micropipette 1000ml and 500ml   [ conical flask 250ml erlenmeyer,5ml -5000ml conical flask,100ml conical flask,conical flask ]
The above items are all exposed to ultraviolet light for 30 minutes.
In addition, prepare gloves, caps, masks, 24-well culture plates, centrifuges + a small lock, unfrozen medicines, DMEM, HCL, NaHCO3, and calf serum.
Plug in the microscope, centrifuge, and magnetic stirrer in advance. Check whether there is alcohol in the alcohol lamp, and whether the iodine and alcohol cotton balls are sufficient. Pass the items in first, then wear a coat, hat and mask. Then turn on the alcohol lamp, arrange the bottles and dishes, and use a bottle and dish to fill the alcohol cotton ball.[ glass lab beaker,beakers glassware,beaker 2000ml,laboratory beaker ]