We can use measuring pipette like this
When using a graduated pipette, insert the tip of the pipe about 1cm below the liquid surface, and the scale on the measuring pipette faces the operator. Use a rubber ball to absorb the liquid to a little more on the required volume mark, quickly block the nozzle with your index finger, and remove the measuring pipette. To the liquid level, vertically sip the tube, gently relax your index finger, slowly put the excess solution back into the reagent bottle, wait until the lowest point of the liquid meniscus in the sipper tube is exactly tangent to the marking line of the required amount, and press the tube mouth with your index finger , Let the tip of the tube lightly touch and stop on the bottle wall, wait for the liquid adhering on the outer wall of the measuring pipette to flow into the bottle, then move the measuring pipette to the container, loosen your index finger, and let the liquid in the straw flow out naturally.
There are many measuring pipette specifications. According to different pipetting volumes, it is very important to select the appropriate measuring pipette. For example, use a 10ml pipette to suck 0.1ml solution or take 1.0ml liquid and use a 0.5ml measuring pipette twice. The correct selection of pipette transfer methods will artificially increase the analysis error and cause the experimental results to be inaccurate. To
In addition, the accuracy of the different sections of the measuring pipette is also different. Generally, the measurement accuracy of the lower part of the measuring pipette is worse than that of the upper part of the measuring pipette, so avoid using the lower part of the straw as much as possible. If you draw 0.6ml of solution, you can use a 1.0ml measuring pipette, draw the solution to 1.0ml, put the solution to 0.4ml, collect the solution that is the desired solution, and discard the remaining 0.4ml solution.
Post time: Nov-05-2021
