Experimental instruments and equipment needed for crude extraction and identification of DNA
[Purpose Requirements]
Learn the methods of crude extraction and identification of DNA, and observe the extracted DNA material.
[Experimental principle]
The solubility of DNA in sodium chloride solution changes with the concentration of sodium chloride. When the concentration of sodium chloride is 0.14 mol/L, the solubility of DNA is the lowest. Using this principle, the DNA dissolved in the sodium chloride solution can be precipitated.
DNA is not soluble in alcohol solution, but certain substances in cells can be soluble in alcohol solution. Using this principle, DNA with less impurities can be further extracted.
DNA will be stained blue when encountering diphenylamine (boiling water bath). Therefore, diphenylamine can be used as a reagent for DNA identification.
[Experimental Instruments and Equipment]
1. Iron frame
2. Glass rod (1 piece)
3. Filter paper
4. Measuring cylinder (100ml, 1 piece) [ laboratory measuring cylinder,Measuring Cylinder 1000ml ]
5. Beaker (1 for 100ml, 2 for each of 50ml and 500ml)
6. Test tube (20ml, 2 pcs) [ borosilicate test tube,customized glass test tube ]
7. Funnel (1 pcs) [ Seperatory Funnel,funnel filter,funnel with filter ]
8. Test tube clamps (2 pcs)
9. Gauze (15 pieces) 10. Centrifuge (1 set)
[ Experimental Materials ]
1. Chicken blood cell fluid (5ml~10ml) 2. 95% ethanol (pre-cooling for 24h)
3. Distilled water 4. Sodium citrate (mass concentration 0.1g/ml)
5. Sodium chloride (2mol/L and 0.015mol/L) 6. Diphenylamine
7. Perchloric acid 8. Acetaldehyde
9. Glacial acetic acid
Post time: Nov-18-2021
